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Colony-forming assays {#s4k}
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MeWo cells were seeded in triplicate in 6-well plates at a density of 2×10^4^ cells/well. After transfection, the cells were cultured in RPMI1640 (HyClone, Logan, UT, USA) containing 10% fetal bovine serum, 2 mM L-glutamine, antibiotics, and antimycotic at 37°C in 5% CO~2~. The culture medium was changed every 2–3 days. After 12–15 days, the colonies in the adherent cell monolayer were fixed with 100% methanol, stained with 0.1% crystal violet, and the number of stained colonies was counted. All assays were performed in triplicate and repeated at least three times. For quantification of the colony size, the area of each colony was estimated using the ‘Measure’ tool in Image J, and the relative area of each colony was calculated
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